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제목 나균에서의 acridine orange 형광염색
저자 김종필, 이민석, 강정원 소속 한국한센복지협회
년도 2013 1
46 번호 1
시작페이지 1 끝페이지 14
첨부 2013-1나학회지2013 - 최종.pdf
요약 Acridine orange stain of Mycobacterium leprae

Jong-Pill Kim, Min-Seok Lee, Jeoung-Won Kang

Institute for Leprosy Research, Korean Hansen Welfare Association

The Ziehl-Neelson’s AFB staining method was mainly used for the AFB observation of the diagnosis of leprosy. However, the fluorescent stain performs better and allows the detection of more positive smears. The limitation for its widespread use has been the high cost for fluorescent microscopes. Novel light-emitting diodes (LED) are inexpensive solutions for fluorescent microscopes, and thus fluorescent stain may be a cost-effective step to improve the diagnosis of leprosy in resource-poor countries. And the comparison of auramine and acridine orange for staining of acid-fast bacteria was showed significantly more acid-fast rods after using acridine orange and the number of "false positive" results was somewhat higher on auramine staining. So acridine orange offers a good alternative to auramine which is considered carcinogenic.

This study evaluated the comparison of the Ziehl-Neelson‘s AFB stain and the acridine orange stain in the skin smear based on PCR. As PCR results were taken as gold standard, results of the study revealed that the sensitivity of Ziehl-Neelson‘s AFB stain was 50% and that of acridine orange stain was 92.2%. This study confirmed that the fluorescence stain method is more sensitive than the Ziehl-Neelsen’s staining method. It is suggested that the training of laboratory technicians on fluorescence microscopy should be scaled up for increased disease control.
key Word : Acridine orange stain, Mycobacterium leprae

교신저자 : 김종필